Papillomavirus typing was carried out by HPV DNA dot and Southern blot hybridization using mixed HPV 6 11, 16 18, and 2 3 DNA. Brazil. Historical and commonly used quantitation methods include the following: Yield gels; Spectrophotometry; Fluorometry; Slot blot hybridization; AluQuant®. This DIGlabeled probe was capable of detecting viral copies of purified OvHV-2 DNA by DBH. In two, the source was unclear. R. Slot blot hybridization was the most commonly used method until recently. . Double-stranded mtDNA, estimated by slot-blot hybridization and real time PCR and expressed as mtDNA-to-nuclear DNA. Autores: L. Hudlow. Klevan, B. The majority of laboratories used the commercially available QuantiBlot® kit. Budowle, W. Using a CCD camera imaging system as a recording device to quantify human DNA by slot blot hybridization. RNA slot-blot hybridization of two cotton cultivars, Giza 45 and Giza 84, performed by use of their respective related probes G45 an d G84 probes. Dot-blot hybridization analysis of subtracted fragments membranes were screened by hybridization with genomic DNA from the tester (panel A) and the driver. A non-radioactive dot-blot nucleic acid hybridization method was evaluated for detecting citrus leaf blotch virus (CLBV, Flexiviridae: Citrivirus). On the other hand, PCR/ DBH was more sensitive than either PCR or. DNA (mtDNA) content remains unexplored.