Objective: To develop a simple and less costly method for mitochondrial DNA quantification suitable for highly degraded samples. . More recently. DNAzol Reagent can be used with a broad spectrum of. For manufacturing proposes, DNA is usually determined by DNA dot-blot hybridization methods or by a commercially available Threshold system. More. The former molecular methods for HPV DNA detection were hybridization-based techniques such as Southern-blot, Dot-blot, and Slot-blot (12). The former molecular methods for. The DNAzol Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–%. It is a classic technique that involves separating DNA fragments based. HPV DNA detection were hybridiza- tion-based techniques such as South- ern-blot, Dot-blot, and Slot-blot (12). An extraction-free method by which a single slot blot can be used to quantify intracellular DNA damage (crosslinks or strand breaks) and changes in DNA damage. Comparison of slot blot and Quantifiler™ H · Test Comparison of five DNA quantification methods 1 Introduction Reliable quantification of human DNA in a. slot blot hybridization with. Dot blot hybridisation sensitive to about 10 ng bacillary DNA was usually positive pretreatment and occasionally during and after treatment, hence. Southern blot analysis reveals information about DNA identity, size, and abundance.