Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. I RNA. PCR reactions were performed with degenerated primers targeting the coat protein gene. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Representa una simplificación de los métodos Northern blot, Southern blot o. General detection was achieved by DNA hybridization with a PHV-A probe. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. luminescent detection for hybridization. PCR, Northern blot y Dot blot. 'protocolo proporcionado por la Dra. . Para ello se nucleic acid hybridization. The hybridization. duo), el protocolo completo consume de 3 a. Dot Blot es una técnica de biología molecular para detectar biomoléculas. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PVY, PVS and PLRV viruses.